The results of the three-year study (2007–2009) concerning the definition of the dynamics of accumulation of photosynthetic pigments in plants of spring triticale of variety of Loaf Kharkovskiy depending on fertilizing with urea and micronutrients are showed. In was found during the experiment a significant effect of trophic and abiotic factors on both the content and the ratio of photosynthetic pigments in the leaves of plants. Proved the advantage of integrated application of urea (Nм 30 kg/ha) and Kristalon to increase the content of chlorophyll and carotenoids in the leaves of plants. Was defined a direct correlation dependence between the content of pigments in leaves and leaf area index.