3d fluorescence microscopy with diffusercam

G Kuo, N Antipa, R Ng, L Waller - Computational Optical Sensing …, 2018 - opg.optica.org
Computational Optical Sensing and Imaging, 2018opg.optica.org
… Abstract: We propose a lensless diffuser-based microscope for 3D fluorescence
microscopy from a single exposure. We use compressed sensing and a local convolution
model to account for the system’s spatially-varying point spread functions in a
computationally efficient manner. … Since most samples are volumetric, 3D imaging is
desirable; however, this generally requires some form of scanning (such as in two-photon or
light sheet microscopy) which limits temporal resolution. DiffuserCam [1] is a recent lensless …
Abstract
We propose a lensless diffuser-based microscope for 3D fluorescence microscopy from a single exposure. We use compressed sensing and a local convolution model to account for the system’s spatially-varying point spread functions in a computationally efficient manner.
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