A β-catenin identified by functional rather than sequence criteria and its role in Wnt/MAPK signaling

AR Kidd, JA Miskowski, KR Siegfried, H Sawa… - Cell, 2005 - cell.com
AR Kidd, JA Miskowski, KR Siegfried, H Sawa, J Kimble
Cell, 2005cell.com
Wnt/MAPK signaling is a common variant of Wnt signaling in C. elegans and has been
implicated in vertebrates. The sys-1 gene works with Wnt/MAPK signaling to control cell
fates during C. elegans development. We report that the SYS-1 amino acid sequence is
novel but that SYS-1 functions as β-catenin: SYS-1 rescues a bar-1/β-catenin null mutant,
binds the POP-1/TCF β-catenin binding domain, and coactivates POP-1-dependent
transcription. Moreover, we provide genetic and molecular evidence that SYS-1 levels are …
Summary
Wnt/MAPK signaling is a common variant of Wnt signaling in C. elegans and has been implicated in vertebrates. The sys-1 gene works with Wnt/MAPK signaling to control cell fates during C. elegans development. We report that the SYS-1 amino acid sequence is novel but that SYS-1 functions as β-catenin: SYS-1 rescues a bar-1/β-catenin null mutant, binds the POP-1/TCF β-catenin binding domain, and coactivates POP-1-dependent transcription. Moreover, we provide genetic and molecular evidence that SYS-1 levels are crucial to POP-1 activity. Our results suggest that Wnt/MAPK signaling promotes POP-1 export from the nucleus to accommodate the limiting availability of its SYS-1/β-catenin transcriptional coactivator. Discovery of SYS-1/β-catenin extends our definition of β-catenins and brings together aspects of the canonical mechanism for Wnt signaling with the noncanonical Wnt/MAPK mechanism. We discuss the idea that a similar pathway may be employed broadly in animal development.
cell.com
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