This article investigates and analyzes the implementation and effectiveness of a Duration-of-Stay torage policy in the Physical Internet. Rather than being stored according to SKU-based information, containers in the Physical Internet are able to gain efficiency through storage according to their length of stay in the warehouse. Data and observations come from different given levels of expected throughput, variance in throughput, and number of storage locations within a unit-load warehouse. The level of efficiency within the system proves to be directly dependent on these different inputs. Whereas an increase in the expected throughput will yield an increase in the overall travel within the system, an increase in the variance of the throughput will yield a similar result. The findings in this paper aid not only operational decisions within the warehouse but also tactical decisions.