This study was carried out for two seasons (2011 and 2012) to evaluate the effectiveness of pneumatic comb harvesting machine and compare it with traditional methods for olive harvesting. This research was conducted at Raba Research Station, Al-Karak Governorate, Jordan, using two olive cultivars Nabali Baladi and Nabali Mohassan. Three methods of harvesting were used: hand harvesting, plastic combs and pneumatic comb machine. Results showed that mechanical harvesting increased significantly the harvesting productivity by five to six times for both cultivars when compared with traditional method (hand harvesting), and increased by two times when compared with harvesting with plastic combs. The percentage of harvested fruits was more than 95% using hand harvesting and ranged from 86%-88% using pneumatic comb machine for both cultivars and the difference was significant between the two harvesting methods. Percentage of detached leaves was about 9-15% with mechanical harvesting by pneumatic comb machine for the two cultivars and between 4-5% with manual harvesting methods. The percentage of injured fruits was less than 2% in Nabali Baladi and Nabali Mohassan in the two seasons, and it increased to 6-7% with mechanical harvesting by pneumatic comb machine. Mechanical harvesting using pneumatic comb machine for Nabali Baladi and Nabali Mohassan cultivars significantly improved the harvesting productivity and reduce the cost of labors.