A fast PCR-based method for the characterization of prophage profiles in strains of the Lactobacillus casei group
D Zaburlin, DJ Mercanti, A Quiberoni - Journal of virological methods, 2017 - Elsevier
Lysogeny is widespread among Lactobacillus strains of the casei group (L. casei, L.
paracasei and L. rhamnosus), and prophages account for most strain‐specific DNA.
Numerous PCR based methods have been developed to detect free phages of lactic acid
bacteria, but they do not take in consideration prophages. In this study, a new PCR method
for the detection of lysogeny was developed using genome sequences of L. casei group
strains (including BL23) and bacteriophages. Nine pairs of primers were designed to …
paracasei and L. rhamnosus), and prophages account for most strain‐specific DNA.
Numerous PCR based methods have been developed to detect free phages of lactic acid
bacteria, but they do not take in consideration prophages. In this study, a new PCR method
for the detection of lysogeny was developed using genome sequences of L. casei group
strains (including BL23) and bacteriophages. Nine pairs of primers were designed to …
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