A novel selectable marker based on Aspergillus niger arginase expression
K Dave, M Ahuja, TN Jayashri, RB Sirola… - Enzyme and microbial …, 2012 - Elsevier
Selectable markers are valuable tools in transforming asexual fungi like Aspergillus niger.
An arginase (agaA) expression vector and a suitable arginase-disrupted host would define a
novel nutritional marker/selection for transformation. The development of such a marker was
successfully achieved in two steps. The single genomic copy of A. niger arginase gene was
disrupted by homologous integration of the bar marker. The agaA disruptant was
subsequently complemented by transforming it with agaA expression vectors. Both citA and …
An arginase (agaA) expression vector and a suitable arginase-disrupted host would define a
novel nutritional marker/selection for transformation. The development of such a marker was
successfully achieved in two steps. The single genomic copy of A. niger arginase gene was
disrupted by homologous integration of the bar marker. The agaA disruptant was
subsequently complemented by transforming it with agaA expression vectors. Both citA and …
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