Recombinant PCR has been used to generate linear fragments for promoter replacement by
transformation in Aspergillus nidulans. A cassette vector carrying the pyr-4 non-homologous
selectable marker and conditional promoter Pr-alcA was constructed for use as a template
for PCR, and is suitable for testing the function of essential genes. Two genes involved in
polar growth, cotA and bemA, were used to assess the system. Efficient targeting was
possible with both genes using approximately 500bp of flanking homologous sequence …