A relic S‐RNase is expressed in the styles of self‐compatible Nicotiana sylvestris
JF Golz, AE Clarke, E Newbigin… - The Plant …, 1998 - Wiley Online Library
The Plant Journal, 1998•Wiley Online Library
We surveyed ribonuclease activity in the styles of Nicotiana spp. and found little or no activity
in self‐compatible species and in a self‐compatible accession of a self‐incompatible
species. All self‐incompatible species had high levels of ribonuclease activity in their style.
Interestingly, one self‐compatible species, N. sylvestris, had a level of stylar ribonuclease
activity comparable to that of some self‐incompatible Nicotiana species. A ribonuclease with
biochemical properties similar to those of the self‐incompatibility (S‐) RNases of N. alata …
in self‐compatible species and in a self‐compatible accession of a self‐incompatible
species. All self‐incompatible species had high levels of ribonuclease activity in their style.
Interestingly, one self‐compatible species, N. sylvestris, had a level of stylar ribonuclease
activity comparable to that of some self‐incompatible Nicotiana species. A ribonuclease with
biochemical properties similar to those of the self‐incompatibility (S‐) RNases of N. alata …
Summary
We surveyed ribonuclease activity in the styles of Nicotiana spp. and found little or no activity in self‐compatible species and in a self‐compatible accession of a self‐incompatible species. All self‐incompatible species had high levels of ribonuclease activity in their style. Interestingly, one self‐compatible species, N. sylvestris , had a level of stylar ribonuclease activity comparable to that of some self‐incompatible Nicotiana species. A ribonuclease with biochemical properties similar to those of the self‐incompatibility (S‐)RNases of N. alata was purified from N. sylvestris styles. The N‐terminal sequence of this protein was used to confirm the identity of a cDNA corresponding to the stylar RNase. The amino acid sequence deduced from the cDNA was related to those of the S‐RNases and included the five conserved regions characteristic of these proteins. It appears that the N. sylvestris RNase may have evolved from the S‐RNases and is an example of a ‘relic S‐RNase’. A number of features distinguish the N. sylvestris RNase from the S‐RNases, and the role these may have played in the presumed loss of the self‐incompatibility response during the evolution of this species are discussed.
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