A resource of targeted mutant mouse lines for 5,061 genes
MC Birling, A Yoshiki, DJ Adams, S Ayabe… - Nature …, 2021 - nature.com
MC Birling, A Yoshiki, DJ Adams, S Ayabe, AL Beaudet, J Bottomley, A Bradley, SDM Brown…
Nature genetics, 2021•nature.com… Despite these efforts, we found that additional QC on the mouse lines themselves was
required to ensure that all IMPC lines resulted from the transmission of the correctly targeted
allele 19 (Supplementary Fig. 4). This additional QC at the mouse level identified a small but
substantial proportion of incorrect alleles that transmitted through the germlines of chimeric mice
derived from clones that had passed initial and secondary validation QC testing in ES cells.
Our experience highlights the importance of careful allele validation before and after … The …
required to ensure that all IMPC lines resulted from the transmission of the correctly targeted
allele 19 (Supplementary Fig. 4). This additional QC at the mouse level identified a small but
substantial proportion of incorrect alleles that transmitted through the germlines of chimeric mice
derived from clones that had passed initial and secondary validation QC testing in ES cells.
Our experience highlights the importance of careful allele validation before and after … The …
The International Mouse Phenotyping Consortium reports the generation of new mouse mutant strains for more than 5,000 genes, including 2,850 novel null, 2,987 novel conditional-ready and 4,433 novel reporter alleles.
nature.com
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