A structure-function study of the C2 domain of cytosolic phospholipase A2: identification of essential calcium ligands and hydrophobic membrane binding residues
The C2 domain of cytosolic phospholipase A 2 (cPLA 2) is involved in the Ca 2+-dependent
membrane binding of this protein. To identify protein residues in the C2 domain of cPLA 2
essential for its Ca 2+ and membrane binding, we selectively mutated Ca 2+ ligands and
putative membrane-binding residues of cPLA 2 and measured the effects of mutations on its
enzyme activity, membrane binding affinity, and monolayer penetration. The mutations of
five Ca 2+ ligands (D40N, D43N, N65A, D93N, N95A) show differential effects on the …
membrane binding of this protein. To identify protein residues in the C2 domain of cPLA 2
essential for its Ca 2+ and membrane binding, we selectively mutated Ca 2+ ligands and
putative membrane-binding residues of cPLA 2 and measured the effects of mutations on its
enzyme activity, membrane binding affinity, and monolayer penetration. The mutations of
five Ca 2+ ligands (D40N, D43N, N65A, D93N, N95A) show differential effects on the …
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