The present study was trying to profile the Romanian Biotech Entrepreneur skills, knowledge and competencies adapted to the national environment in the European context. The study is based on a questionnaire survey applied on a panel of 30 Romanian entrepreneurs or top-management persons in the field of biotechnology. The set of 50 questions have covered different topics, respectively the general profile of the respondents and their companies, entrepreneurial profiling from the respondents’ point of view or the entrepreneurial societal context. It was concluded that the intervention areas would be the following: much more measures to support young entrepreneurs, less bureaucracy and taxes, changes in work legislation, professionalism in funding agencies, more training opportunities and written guides, changes of the negative society’s perception about the entrepreneurship. An important conclusion of the study was that the training, especially the high-education, is an important factor for starting and running a business in the biotech field.