… of epo using either radioactive reagents or labeled epo. Epo labeled with tritium at the sialic
acid … Under the same conditions, (37 C, 20 min) endoproteinase Arg-C did not hydrolyze epo …

… Fully desialatd erythropoietin, whether Qesialated by mild acid hydrolysis or sialidase,
invariably focused as three components at pH 4.7, 5.1, and 6.1 (f 0.1 pH unit). Recovery of activity …

… Since we found the increase in activity even when desialation was done by acid hydrolysis
… of marrow cells with sialidase does not cause any change in their response to erythropoietin…

… Oligosaccharides were subjected to acid hydrolysis in the presence of 2 M HCl in sealed
glass tubes at 100 C for 2 h. After drying in a vacuum, the resulting monosaccharides were …

… In this study, efforts were made to optimize the conditions for the hydrolysis of EPO since the
efficiency of protein hydrolysis is dependent on a number of factors, including the presence …

… These findings do not snpport a concept that erythropoietin behaves as a ‘carrier’ protein.
Since the neuraminic acid of erythropoietin is labile to very mild acid hydrolysis, and its …

… extensive works on erythropoietin and erythropoiesis have been the books published by
Krantz and … removal of sialic acid. On the other … form of erythropoietin retains its activity …

… result of a few minutes of erythropoietin action is not restricted to the … On reexamining the
problem we find that erythropoietin … All steps, exceptthe DNase hydrolysis, were carried out at 0-…

… Amino acid analyses were carried out by hydrolysis for 16 h in 6 N hydrochloric acid in a
sealed … The effect of proteolytic hydrolysis upon Step 4 B erythropoietin is shown in Fig. 3. Both …

… human erythropoietin and 125I-labeled, desialylated recombinant erythropoietin. The
immediate volume of distribution of 125I-labeled, recombinant erythropoietin approximated that of …