Acquisition and transmission of Theileria parva by vector tick, Rhipicephalus appendiculatus

S Konnai, S Imamura, C Nakajima, WH Witola… - Acta tropica, 2006 - Elsevier
In order to investigate the transmission dynamics of Theileria parva (T. parva) by the brown
ear tick, Rhipicephalus appendiculatus (R. appendiculatus), under experimental conditions,
detection of T. parva in ticks and cattle was performed by a quantitative real-time PCR assay.
A calf inoculated with a T. parva mixture became PCR-positive for T. parva infection on day 8
post-inoculation, and subsequently, nymphal ticks were introduced and maintained to feed
on the infected calf for 6 days. Engorged nymphs were collected daily and allowed to molt …
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