Activated charcoal—a potential material in glucoamylase recovery
SO Kareem, I Akpan, TOS Popoola… - Enzyme Research, 2011 - Wiley Online Library
Enzyme Research, 2011•Wiley Online Library
The potential of activated charcoal in the purification of fungal glucoamylase was
investigated. Various concentrations of activated charcoal (1–4% w/v) were used to
concentrate crude glucoamylase from Rhizopus oligosporus at different temperature values
(30–50° C). Effects of pH (3.0–6.0) and contact time (0–60 min) on enzyme purification were
also monitored. Activated charcoal (3% w/v) gave a 16‐fold purification in a single‐step
purification at 50° C for 20 min and pH 5.5. The result of SDS‐PAGE analysis of purified …
investigated. Various concentrations of activated charcoal (1–4% w/v) were used to
concentrate crude glucoamylase from Rhizopus oligosporus at different temperature values
(30–50° C). Effects of pH (3.0–6.0) and contact time (0–60 min) on enzyme purification were
also monitored. Activated charcoal (3% w/v) gave a 16‐fold purification in a single‐step
purification at 50° C for 20 min and pH 5.5. The result of SDS‐PAGE analysis of purified …
The potential of activated charcoal in the purification of fungal glucoamylase was investigated. Various concentrations of activated charcoal (1–4% w/v) were used to concentrate crude glucoamylase from Rhizopus oligosporus at different temperature values (30–50°C). Effects of pH (3.0–6.0) and contact time (0–60 min) on enzyme purification were also monitored. Activated charcoal (3% w/v) gave a 16‐fold purification in a single‐step purification at 50°C for 20 min and pH 5.5. The result of SDS‐PAGE analysis of purified glucoamylase showed two major protein bands with corresponding molecular weight of 36 kDa and 50 kDa. The method is inexpensive, rapid, and simple which could facilitate downstream processing of industrial enzyme.
Wiley Online Library以上显示的是最相近的搜索结果。 查看全部搜索结果