[PDF][PDF] Agrobacterium mediated transfer of nptII and gus genes in Camellia assamica
N Bhattacharyya, HR Singh… - Journal of …, 2014 - academicjournals.org
The present work is an attempt to develop transgenic hairy root system in Camellia
assamica, using leaves infected by Agrobacterium rhizogenes strain LBA9402, harboring
binary vector (pART27 and pBI121) carrying β-glucuronidase (gus) as reporter gene and
neomycin phosphotransferase (nptII) as selection marker. The transformed hairy roots were
grown in phytohormone free MS media in the presence of kanamycin 50 µg/ml. The
incorporation and expression of foreign genes were checked by initial gus assay followed by …
assamica, using leaves infected by Agrobacterium rhizogenes strain LBA9402, harboring
binary vector (pART27 and pBI121) carrying β-glucuronidase (gus) as reporter gene and
neomycin phosphotransferase (nptII) as selection marker. The transformed hairy roots were
grown in phytohormone free MS media in the presence of kanamycin 50 µg/ml. The
incorporation and expression of foreign genes were checked by initial gus assay followed by …
Agrobacterium mediated transfer of nptII and gus genes in Camellia assamica.
NB Neelakshi Bhattacharyya, HR Singh… - 2014 - cabidigitallibrary.org
The present work is an attempt to develop transgenic hairy root system in Camellia
assamica, using leaves infected by Agrobacterium rhizogenes strain LBA9402, harboring
binary vector (pART27 and pBI121) carrying β-glucuronidase (gus) as reporter gene and
neomycin phosphotransferase (npt II) as selection marker. The transformed hairy roots were
grown in phytohormone free MS media in the presence of kanamycin 50 µg/ml. The
incorporation and expression of foreign genes were checked by initial gus assay followed by …
assamica, using leaves infected by Agrobacterium rhizogenes strain LBA9402, harboring
binary vector (pART27 and pBI121) carrying β-glucuronidase (gus) as reporter gene and
neomycin phosphotransferase (npt II) as selection marker. The transformed hairy roots were
grown in phytohormone free MS media in the presence of kanamycin 50 µg/ml. The
incorporation and expression of foreign genes were checked by initial gus assay followed by …
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