Agrobacterium-mediated transformation of Zymoseptoria tritici
H Child, N Helmstetter - 2022 - protocols.io
H Child, N Helmstetter
2022•protocols.ioThis protocol describes the procedure for transforming plasmids into the plant pathogenic
fungus Zymoseptoria tritici by Agrobacterium tumefaciens-mediated transformation. It is
based on the protocol first described by Zwiers and De Waard (2001; doi: 10.1007/
s002940100216) and utilises A. tumefaciens ternary vectors developed by Sidhu et
al.(2015a; doi: 10.1016/j. fgb. 2015.04. 015), Sidhu et al.(2015b; doi: 10.1016/j. fgb. 2015.03.
021) and Kilaru et al.(2015; doi: 10.1016/j. fgb. 2015.03. 018).
fungus Zymoseptoria tritici by Agrobacterium tumefaciens-mediated transformation. It is
based on the protocol first described by Zwiers and De Waard (2001; doi: 10.1007/
s002940100216) and utilises A. tumefaciens ternary vectors developed by Sidhu et
al.(2015a; doi: 10.1016/j. fgb. 2015.04. 015), Sidhu et al.(2015b; doi: 10.1016/j. fgb. 2015.03.
021) and Kilaru et al.(2015; doi: 10.1016/j. fgb. 2015.03. 018).
Abstract
This protocol describes the procedure for transforming plasmids into the plant pathogenic fungus Zymoseptoria tritici by Agrobacterium tumefaciens-mediated transformation. It is based on the protocol first described by Zwiers and De Waard (2001; doi: 10.1007/s002940100216) and utilises A. tumefaciens ternary vectors developed by Sidhu et al.(2015a; doi: 10.1016/j. fgb. 2015.04. 015), Sidhu et al.(2015b; doi: 10.1016/j. fgb. 2015.03. 021) and Kilaru et al.(2015; doi: 10.1016/j. fgb. 2015.03. 018).
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