characterizing functions of gene products in planta. However, the practicability of the method
in the model liverwort Marchantia polymorpha has not yet been thoroughly described. Here
we report a simple and robust method for Agrobacterium-mediated transient transformation
of Marchantia thalli and its applicability. When thalli of M. polymorpha were co-cultured with
Agrobacterium tumefaciens carrying β-glucuronidase (GUS) genes, GUS staining was …