Agrobacterium-Mediated Transient Transformation of Marchantia Liverworts

H Iwakawa, K Melkonian, T Schlüter… - Plant and Cell …, 2021 - academic.oup.com
Agrobacterium-mediated transient gene expression is a rapid and useful approach for
characterizing functions of gene products in planta. However, the practicability of the method
in the model liverwort Marchantia polymorpha has not yet been thoroughly described. Here
we report a simple and robust method for Agrobacterium-mediated transient transformation
of Marchantia thalli and its applicability. When thalli of M. polymorpha were co-cultured with
Agrobacterium tumefaciens carrying β-glucuronidase (GUS) genes, GUS staining was …

Agrobacterium-Mediated Transient Transformation of Marchantia Liverworts

K Melkonian, T Schlüter, H Iwakawa, R Nishihama… - (No Title), 2021 - cir.nii.ac.jp
< jats: title> Abstract</jats: title>< jats: p>< jats: italic> Agrobacterium</jats: italic>-mediated
transient gene expression is a rapid and useful approach for characterizing functions of
gene products< jats: italic> in planta</jats: italic>. However, the practicability of the method
in the model liverwort< jats: italic> Marchantia polymorpha</jats: italic> has not yet been
thoroughly described. Here we report a simple and robust method for< jats: italic>
Agrobacterium</jats: italic>-mediated transient transformation of< jats: italic> …
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