An alternative simple method for mass production of chimeric embryos by coculturing denuded embryos and embryonic stem cells in Eppendorf vials
KH Lee, C Chuang, HW Wang, L Stone, CH Chen… - Theriogenology, 2007 - Elsevier
The generation of germline competent chimeric mice via embryonic stem (ES) cells is a
crucial step in developing gene-manipulated mouse models. To date, techniques for
generating chimeric mice include direct microinjection of ES cells into the cavity of 3.5-d post-
coitum (dpc) blastocysts and aggregating or coculturing 2.5 dpc zona pellucida-free
(denuded) embryos with ES cells. We present here a procedure that is simple and
reproducible for mass producing (10–150 embryos/vial/time) chimeric embryos by …
crucial step in developing gene-manipulated mouse models. To date, techniques for
generating chimeric mice include direct microinjection of ES cells into the cavity of 3.5-d post-
coitum (dpc) blastocysts and aggregating or coculturing 2.5 dpc zona pellucida-free
(denuded) embryos with ES cells. We present here a procedure that is simple and
reproducible for mass producing (10–150 embryos/vial/time) chimeric embryos by …
An alternative simple method for mass production of chimeric embryos by coculturing denuded embryos and embryonic stem cells in Eppendorf vials.
LKH Lee KunHsiung, CCK Chuang ChinKai… - 2007 - cabidigitallibrary.org
The generation of germline competent chimeric mice via embryonic stem (ES) cells is a
crucial step in developing gene-manipulated mouse models. To date, techniques for
generating chimeric mice include direct microinjection of ES cells into the cavity of 3.5-d post-
coitum (dpc) blastocysts and aggregating or coculturing 2.5 dpc zona pellucida-free
(denuded) embryos with ES cells. We present here a procedure that is simple and
reproducible for mass producing (10-150 embryos/vial/time) chimeric embryos by …
crucial step in developing gene-manipulated mouse models. To date, techniques for
generating chimeric mice include direct microinjection of ES cells into the cavity of 3.5-d post-
coitum (dpc) blastocysts and aggregating or coculturing 2.5 dpc zona pellucida-free
(denuded) embryos with ES cells. We present here a procedure that is simple and
reproducible for mass producing (10-150 embryos/vial/time) chimeric embryos by …
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