[PDF][PDF] An efficient method for DNA extraction from paraffin wax embedded tissues for PCR amplification of human and viral DNA
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Background and Objective: Formalin-fixed paraffin-embedded tissues are a valuable source
of DNA for molecular studies. We designed and optimized an efficient procedure for DNA
extraction from formalin-fixed paraffin embedded tissues. Materials and Methods: Seventy
three blocks of cervical paraffin-embedded tissues were investigated. DNA was extracted
using 45 minutes boiling in alkaline solution together with 10 beads of Chelex-20, followed
by phenol-chloroform extraction and alcohol precipitation. Results: This method produced …
of DNA for molecular studies. We designed and optimized an efficient procedure for DNA
extraction from formalin-fixed paraffin embedded tissues. Materials and Methods: Seventy
three blocks of cervical paraffin-embedded tissues were investigated. DNA was extracted
using 45 minutes boiling in alkaline solution together with 10 beads of Chelex-20, followed
by phenol-chloroform extraction and alcohol precipitation. Results: This method produced …
Abstract
Background and Objective: Formalin-fixed paraffin-embedded tissues are a valuable source of DNA for molecular studies. We designed and optimized an efficient procedure for DNA extraction from formalin-fixed paraffin embedded tissues.
Materials and Methods: Seventy three blocks of cervical paraffin-embedded tissues were investigated. DNA was extracted using 45 minutes boiling in alkaline solution together with 10 beads of Chelex-20, followed by phenol-chloroform extraction and alcohol precipitation.
Results: This method produced DNA suitable for amplification using primers specific for human SMN and
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