This paper describes an electrochemical method for the preparation of ⁵⁵Fe sources, utilized in the calibration of low energy X-ray detectors. In this process, electrodeposition of ⁵⁵Fe containing 185 MBq (∼5 mCi) of activity on a small circular cross sectional flat surface area (∼0.07 cm²) within a cylindrical Cu metal rod was carried out at a current density of 20 mA/cm² at 18 ºC for 4 h using sulphate bath maintained at pH ∼ 2. The electrolytic cell, the experimental set up used and the process of ⁵⁵Fe deposition were described. The effect of various experimental parameters influencing the electrodeposition of ⁵⁵Fe were identified and optimized. The texture and morphology of the electrodeposited samples were examined by X-ray diffraction (XRD), SEM and EDS techniques. The quantity of deposited ⁵⁵Fe activity was assayed in a calibrated ion chamber. Quality control tests were carried out to ensure nonleachability, uniform distribution of activity and stability of the sources.