Microwave scanning techniques are used intensively in various image reconstruction processes like Microwave Imaging, Microwave Tomography Technique (MTT), and Microwave Remote Sensing etc. Generation of high-resolution images depend on the corresponding beamwidth of the microwave signal used. Researchers are focusing on suitable beamwidth adjustment techniques depending on the respective application areas. In this paper an analysis has been made for a narrow beamwidth microwave scanning technique which is very essential to make high resolution images. Especially in case of biological targets, it is necessary to use a narrow beam that is comparable with the small cell size. Here two different techniques have been shown on two different biological targets. The first one where the narrow beamwidth has been generated keeping the operating frequency fixed at 2.4 GHz and varying the number of dipole array antenna elements. This method may be applicable in MTT for cancerous cell detection in human body. In the second method, the frequency of operation has been selected at 5 GHz and it may be helpful in brain stroke detection using MTT. Further we have extended the analysis of beamwidth by keeping the number of array elements constant while changing the frequency to observe the effects of frequency in narrow beamwidth generation. Finally, it has been seen that both of these techniques are found to be very much efficient for narrow beamwidth microwave scanning of various biomedical targets.