The phenomenon of urban population growth is a global concern which will result in a decrease in the value of ecosystem services in an area. Sukoharjo Regency is an area affected by the development of Surakarta City; therefore, rapid growth is taking place. The objective of this study is to investigate the interplay between urbanization, ecosystem services, and the provision of clean water in Sukoharjo Regency in 2022. The methods used in the study were calculating the percentage of the urban population to determine the level of urbanisation, AHP and overlay to ascertain the ecosystem service score, together with cross-tabulation to establish the relationship between these two variables. The result of this study is that the level of urbanisation produces a pattern that districts in the north tend to comprise a higher level. The clean water supply ecosystem services in Sukoharjo Regency obtained results dominated by the low to medium level. The situation regarding the level of urbanisation and ecosystem services in Sukoharjo Regency reveals a relationship where an increase in the level of urbanisation will reduce the value of ecosystem services.