Analysis of bacterial community structures in two sewage treatment plants with different sludge properties and treatment performance by nested PCR-DGGE method
The bacterial community structures in two sewage treatment plants with different processes
and performance were investigated by denaturing gradient gel electrophoresis (DGGE) of
nested polymerase chain reaction (nested PCR) amplified 16S rRNA gene fragments with
group-specific primers. Samples of raw sewage and treated effluents were amplified using
the whole-cell PCR method, and the activated sludge samples were amplified using the
extracted genomic DNA before the PCR products were loaded on the same DGGE gel for …
and performance were investigated by denaturing gradient gel electrophoresis (DGGE) of
nested polymerase chain reaction (nested PCR) amplified 16S rRNA gene fragments with
group-specific primers. Samples of raw sewage and treated effluents were amplified using
the whole-cell PCR method, and the activated sludge samples were amplified using the
extracted genomic DNA before the PCR products were loaded on the same DGGE gel for …
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