Analysis of fecal sludges reveals common enteric pathogens in urban Maputo, Mozambique

D Capone, D Berendes, O Cumming… - … science & technology …, 2020 - ACS Publications
Environmental science & technology letters, 2020ACS Publications
Sewage surveillance is increasingly used in public health applications; metabolites,
biomarkers, and pathogens are detectable in wastewater and can provide useful information
about community health. Work on this topic has been limited to wastewaters in mainly high-
income settings, however. In low-income countries, where the burden of enteric infection is
high, nonsewered sanitation predominates. In order to assess the utility of fecal sludge
surveillance as a tool to identify the most prevalent enteric pathogens circulating among at …
Sewage surveillance is increasingly used in public health applications; metabolites, biomarkers, and pathogens are detectable in wastewater and can provide useful information about community health. Work on this topic has been limited to wastewaters in mainly high-income settings, however. In low-income countries, where the burden of enteric infection is high, nonsewered sanitation predominates. In order to assess the utility of fecal sludge surveillance as a tool to identify the most prevalent enteric pathogens circulating among at-risk children, we collected 95 matched child stool and fecal sludge samples from household clusters sharing latrines in urban Maputo, Mozambique. We analyzed samples for 20 common enteric pathogens via multiplex real-time quantitative PCR. Among the 95 stools matched to fecal sludges, we detected the six most prevalent bacterial pathogens (Enteroaggregative E. coli, Shigella/Enteroinvasive E. coli, Enterotoxigenic E. coli, Enteropathogenic E. coli, shiga-toxin producing E. coli, Salmonella), and all three protozoan pathogens (Giardia duodenalis, Cryptosporidium parvum, Entamoeba histolytica) in the same rank order in both matrices. We did not observe the same trend for viral pathogens or soil-transmitted helminths, however. Our results suggest that sampling fecal sludges from onsite sanitation offers potential for localized pathogen surveillance in low-income settings where enteric pathogen prevalence is high.
ACS Publications
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