This paper analyzes customers’ impatience in Markovian queueing system with multiple working vacations and Bernoulli schedule vacation interruption, where customers’ impatience is due to the servers’ vacation. During the working vacation period, if there are customers in the queue, the vacation can be interrupted at a service completion instant and the server begins a regular busy period with probability 1 − q or continues the vacation with probability q. We obtain the probability generating functions of the stationary state probabilities and deduce the explicit expressions of the system sizes when the server is in a normal service period and in a Bernoulli schedule vacation interruption, respectively. Various performance measures such as the mean system size, the proportion of customers served, the rate of abandonment due to impatience, and the mean sojourn time of a customer served are derived. We obtain the stochastic decomposition structures of the queue length and waiting time. Finally, some numerical results to show the impact of model parameters on performance measures of the system are presented.