In Mediterranean countries, water shortage is becoming a problem of high concern affecting the local economy, mostly based on agriculture. The problem is not only the scarcity of water in terms of average per capita, but the high cost to make water available at the right place, at the right time with the required quality. In these cases, an integrated approach for water resources management including wastewater is required. The management should also include treated wastewater (TWW) reclamation and reuse, especially for agricultural irrigation. In Italy, TWW reuse is regulated by a quite restrictive approach (Ministry Decree, M.D. 185/03), especially for some chemical compounds and microbiological parameters. The aim of the paper is the evaluation of TWW reuse potential in Sicily. A Geographic Information System (GIS) was built at regional level to quantify and locate the available TWW volumes. In particular, the characteristics of wastewater treatment plants (WWTPs) were integrated, through the GIS, with data on irrigation district areas. Moreover, in order to evaluate the Italian approach for reuse practice in agriculture, the water quality of different TWW effluents was analysed on the basis of both the Italian standards and the WHO guidelines.