Analysis on bioactivity of HIV-1 integrase by ELISA method
WH Feng, JS Huang, JB Zhan - Zhejiang da xue xue bao. Yi xue …, 2007 - europepmc.org
WH Feng, JS Huang, JB Zhan
Zhejiang da xue xue bao. Yi xue ban= Journal of Zhejiang University …, 2007•europepmc.orgObjective To develop an ELISA-based method for analyzing biologic activities of HIV-1
integrase and for high throughput screening of integrase inhibitors. Methods After
expression, renaturation and purification of integrase, the bioactivity of integrase and the
inhibition of luffin-a were evaluated with an in vitro assay based on biotin-avidin EILSA and
chemiluminescent substrates.
integrase and for high throughput screening of integrase inhibitors. Methods After
expression, renaturation and purification of integrase, the bioactivity of integrase and the
inhibition of luffin-a were evaluated with an in vitro assay based on biotin-avidin EILSA and
chemiluminescent substrates.
Objective
To develop an ELISA-based method for analyzing biologic activities of HIV-1 integrase and for high throughput screening of integrase inhibitors.
Methods
After expression, renaturation and purification of integrase, the bioactivity of integrase and the inhibition of luffin-a were evaluated with an in vitro assay based on biotin-avidin EILSA and chemiluminescent substrates.
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