The present study details with the antibacterial assay of therapeutic importance from Parquetina nigrescens, a useful medicinal plant. The crude aqueous and ethanol extracts of the plant leaves were assay for antibacterial activity. Some of the test organisms were found resistant to the aqueous extract concentrations while E. coli was found resistant to the ethanol extract in all the concentrations Considered. S. aureus was the most susceptible to the extracts concentrations considered where 100mg/ml of aqueous inhibited the organism with halo> 12mm and> 20mm with 300mg/ml of the ethanol extract. Methycilin the reference antibiotic had between 17-26mm inhibitory zones on the tested organisms. The MIC of the aqueous extract concentration was between 25-50mg/ml while it was between 12.5-50.0 mg/ml with the ethanol extract on the inhibited organisms.