Under five mortality which implies the death of a child before his or her fifth birthday is a major problem in developing countries like Nigeria. Aside other factors; microbial infection is grossly implicated in under five mortality. More problems however are caused by antimicrobial resistant microorganisms. Antimicrobial resistance by bacteria has become a global problem leading to much treatment failure. This study was therefore carried out to ascertain the antimicrobial profile of clinical isolates from pre-nursery school children between the ages of 3 and 5 years with a view to proposing the antibiotic of choice against the common isolates. Known isolates from pre-school children in Nnewi were used for the study. These isolates were subjected to standard microbiological and biochemical protocols to confirm their identities. The disc diffusion method was used to ascertain the susceptibility of the test organisms to commonly used broad spectrum antibiotics. Multidiscs containing ten broad spectrum antibiotics were used. The data obtained from this study were analyzed using the statistical package for social science (SPSS) version 9.5. The mean, range and standard deviation of the variables were determined where applicable. Frequency distribution, percentages and cross tabulation to examine relation between variables was done. The difference between frequencies was tested using Yate’s corrected chi square (χ2) test or Fisher’s exact test. Statistical significance was set at p< 0.05. The overall invitro sensitivity of the isolates was 100% to ciprofloxacin, 90.6% to clindamycin, 68.8% to gentamicin and 59.4% to cefuroxime. Fifty percent of the isolate were sensitive to cephalexim. The isolate were 34.4% sensitive to nitrofuraintoin and nalidixic acid, 25% sensitive to cotrimoxaole and 9.4% sensitive to erythromycin. All isolates were resistant to ampicillin.