In developing countries of tropical regions skin diseases are more common due to mycotic infection. The use of medicinal herbs in the treatment of skin diseases including mycotic infections is an age-old practice in many parts of the world. The drugs which are being used against dermatophytosis exhibits several side effects and have limited efficacy. So that there is a distinct need for the discovery of new safer and more effective antifungal agents. Because herbal remedies used in traditional folk medicine may help to overcome the growing problem of resistance to antifungal drugs and their relative toxicity. In this study, the in-vitro antifungal activity of calotropis plocera leaves extract were evaluated against three different genera of dermatoplytes viz. Microsporium, Trichophyton and Epidermophyton by dilution agar method. Calotropis procera belongs to family Asclepediaceae. Medicinal properties of this plant are antimicrobial, antiinflammatory, analgesic and useful eczema and also for thrush and in mycotic enteritis. The ethanolic extracts from the leaves of this plant inhibited the growth of the dermatophytes tested at a concentration of 250 gml-1. The MFC values of this compound was between 500-1000 gml-1. Based on the results of this study, we can consider calotropis procera extract as a new source of developing local antifungal agents. However, further studies are needed to determine the efficacy of active component of this extract.