Antimicrobial activity of aqueous, methanol and chloroform leaf extracts of Cissus multistriata were investigated against 8 bacterial and 2 fungal test organisms, using the tube dilution and agar ditch diffusion methods. Aqueous leaf extract had no activity against both the bacterial and fungal test organisms. Both the methanol and chloroform leaf extracts inhibited all the test organisms with chloroform leaf extract showing the highest zone of inhibition against Escherichia coli (diameter 25 mm) and least against Staphylococcus aureus (diameter 13 mm). The methanol leaf extract was least inhibitory against Salmonella typhi (diameter 8 mm) and most inhibitory against S. aureus (diameter 15 mm). The methanol leaf extract of C. multistriata show more antifungal activity compared with