Aphid effector Me10 interacts with tomato TFT 7, a 14‐3‐3 isoform involved in aphid resistance
R Chaudhary, HC Peng, J He, J MacWilliams… - New …, 2019 - Wiley Online Library
We demonstrated previously that expression of Macrosiphum euphorbiae salivary protein
Me10 enhanced aphid reproduction on its host tomato (Solanum lycopersicum). However,
the mechanism of action of Me10 remained elusive. To confirm the secretion of Me10 by the
aphid into plant tissues, we produced Me10 polyclonal antibodies. To identify the plant
targets of Me10, we developed a tomato immune induced complementary DNA yeast two‐
hybrid library and screened it with Me10 as bait. Immunoprecipitation and bimolecular …
Me10 enhanced aphid reproduction on its host tomato (Solanum lycopersicum). However,
the mechanism of action of Me10 remained elusive. To confirm the secretion of Me10 by the
aphid into plant tissues, we produced Me10 polyclonal antibodies. To identify the plant
targets of Me10, we developed a tomato immune induced complementary DNA yeast two‐
hybrid library and screened it with Me10 as bait. Immunoprecipitation and bimolecular …
[PDF][PDF] Aphid effector Me10 interacts with tomato TFT7, a 14-3-3 isoform involved in aphid resistance
I Kaloshian - New Phytologist, 2019 - academia.edu
We demonstrated previously that expression of Macrosiphum euphorbiae salivary protein
Me10 enhanced aphid reproduction on its host tomato (Solanum lycopersicum). However,
the mechanism of action of Me10 remained elusive. To confirm the secretion of Me10 by the
aphid into plant tissues, we produced Me10 polyclonal antibodies. To identify the plant
targets of Me10, we developed a tomato immune induced complementary DNA yeast two-
hybrid library and screened it with Me10 as bait. Immunoprecipitation and bimolecular …
Me10 enhanced aphid reproduction on its host tomato (Solanum lycopersicum). However,
the mechanism of action of Me10 remained elusive. To confirm the secretion of Me10 by the
aphid into plant tissues, we produced Me10 polyclonal antibodies. To identify the plant
targets of Me10, we developed a tomato immune induced complementary DNA yeast two-
hybrid library and screened it with Me10 as bait. Immunoprecipitation and bimolecular …
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