Application of SHAPE reveals in vivo RNA folding under normal and growth-stressed conditions in the human parasite Entamoeba histolytica
Selective 2′-hydroxyl acylation analyzed by primer extension (SHAPE) is a versatile
sequence independent method to probe RNA structure in vivo and in vitro. It has so far been
tried mainly with model organisms. We show that cells of Entamoeba histolytica, a protozoan
parasite of humans are hyper-sensitive to the in vivo SHAPE reagent, NAI, and show rapid
loss of viability and RNA integrity. We optimized treatment conditions with 5.8 S rRNA and
Eh_U3 snoRNA to obtain NAI-modification while retaining RNA integrity. The modification …
sequence independent method to probe RNA structure in vivo and in vitro. It has so far been
tried mainly with model organisms. We show that cells of Entamoeba histolytica, a protozoan
parasite of humans are hyper-sensitive to the in vivo SHAPE reagent, NAI, and show rapid
loss of viability and RNA integrity. We optimized treatment conditions with 5.8 S rRNA and
Eh_U3 snoRNA to obtain NAI-modification while retaining RNA integrity. The modification …
以上显示的是最相近的搜索结果。 查看全部搜索结果