Arabidopsis group Ie formins localize to specific cell membrane domains, interact with actin‐binding proteins and cause defects in cell expansion upon aberrant …
The closely related proteins AtFH4 and AtFH8 represent the group Ie clade of Arabidopsis
formin homologues. The subcellular localization of these proteins and their ability to affect
the actin cytoskeleton were examined.• AtFH4 protein activity was identified using
fluorimetric techniques. Interactions between Arabidopsis profilin isoforms and AtFH4 were
assayed in vitro and in vivo using pull‐down assays and yeast‐2‐hybrid. The subcellular
localization of group Ie formins was observed with indirect immunofluorescence (AtFH4) and …
formin homologues. The subcellular localization of these proteins and their ability to affect
the actin cytoskeleton were examined.• AtFH4 protein activity was identified using
fluorimetric techniques. Interactions between Arabidopsis profilin isoforms and AtFH4 were
assayed in vitro and in vivo using pull‐down assays and yeast‐2‐hybrid. The subcellular
localization of group Ie formins was observed with indirect immunofluorescence (AtFH4) and …
[引用][C] Arabidopsis group le formins localize to specific cell membrane domains, interact with actin-binding proteins and cause defects in cell expansion upon aberrant …
MJ DECKS, F CVRCKOVA, LM MACHESKY… - New …, 2005 - pascal-francis.inist.fr
… cell wall cell-cell communication … Cell biochemistry …
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