Bacterial Lipopolysaccharide Plus Interferon-Ɣ Elicit a Very Fast Inhibition of a Ca2+-dependent Nitric-oxide Synthase Activity in Human Astrocytoma Cells
M Colasanti, E Cavalieri, T Persichini, V Mollace… - Journal of Biological …, 1997 - ASBMB
Previous results indicate that induction of inducible nitric-oxide synthase (iNOS) expression
may be kept suppressed by the endogenous NO level as produced by a constitutive NOS
(cNOS) enzyme. In cell types possessing both cNOS and iNOS, this may represent an
evident paradox. Here, we report that lipopolysaccharide and interferon-Ɣ, which are able to
strongly induce iNOS in astrocytoma cells, can rapidly inhibit the NO production generated
by the constitutive NOS isoform, thus obtaining the best conditions for iNOS induction and …
may be kept suppressed by the endogenous NO level as produced by a constitutive NOS
(cNOS) enzyme. In cell types possessing both cNOS and iNOS, this may represent an
evident paradox. Here, we report that lipopolysaccharide and interferon-Ɣ, which are able to
strongly induce iNOS in astrocytoma cells, can rapidly inhibit the NO production generated
by the constitutive NOS isoform, thus obtaining the best conditions for iNOS induction and …