Bacterial expression and antibiotic activities of recombinant variants of human β-defensins on pathogenic bacteria and M. tuberculosis
L Corrales-Garcia, E Ortiz… - Protein expression and …, 2013 - Elsevier
Five variants of human β-defensins (HBDs) were expressed in Escherichia coli using two
vector systems (pET28a (+) and pQE30) with inducible expression by IPTG. The last vector
has not been previously reported as an expression system for HBDs. The recombinant
peptides were different in their lengths and overall charge. The HBDs were expressed as
soluble or insoluble proteins depending on the expression system used, and the final
protein yields ranged from 0.5 to 1.6 mg of peptide/g of wet weight cells, with purities higher …
vector systems (pET28a (+) and pQE30) with inducible expression by IPTG. The last vector
has not been previously reported as an expression system for HBDs. The recombinant
peptides were different in their lengths and overall charge. The HBDs were expressed as
soluble or insoluble proteins depending on the expression system used, and the final
protein yields ranged from 0.5 to 1.6 mg of peptide/g of wet weight cells, with purities higher …