Lithium-ion batteries are a key technology for current and future energy storage, whether they are used for mobile or stationary application. In particular, they play an important role for the electrification of mobility due to their high power and energy density and therefore their battery lifetime prediction is a fundamental aspect for successful market introduction. The ageing data provided by the battery manufacturers result from standard ageing tests. For this reason, it is very important to link, in the way as simple as possible, the effect of driving cycles (urban, extra-urban, combined) with the ageing of the batteries. The aim of this work is to investigate on the effect of driving cycles on the batteries lifetime through tests performed on different cells for different kinds of cycle. Afterwards, simulation tests are made in order to estimate the battery lifetime for different driving cycles of the electric vehicle.