Objective Functional investigation of novel gene/protein targets associated with adipocyte
differentiation or function heavily relies on efficient and accessible tools to manipulate gene
expression in adipocytes in vitro. Recent advances in gene-editing technologies such as
CRISPR-Cas9 have not only eased gene editing but also greatly facilitated modulation of
gene expression without altering the genome. Here, we aimed to develop and validate a
competent in vitro adipocyte model of controllable functionality as well as multiplexed gene …