Aryl heteroaryl carbinol derivatives are significant precursors for production of pharmaceutically prominent substances bearing antihistaminic, anesthetic, diuretic, antidepressive, antiarrhythmic, and anticholinergic effects [1–4]. Large-scale preparation of these alcohols under effective catalytic and enantioselective process is an important task for their production as they should be obtained as enantiomerically pure molecules for drug synthesis. All these requirements especially costs for the separation of two enantiomers have led researcher to explore novel environmental and inexpensive enantioselective production ways [5]. Significant demands for practical, easy, and inexpensive enantioselective syntheses of these compounds have been increasing every day, and thus, the environmentally friendly and cheap enantiopure production of diaryl methanol has been the subject of numerous studies [4]. Recently, several studies reported the production of enantiopure diaryl methanol using either the addition of aryl nucleophiles to aryl aldehyde or the asymmetric reduction of corresponding ketones with chemical catalysts [6–9]. However, this chemical catalyst has suffered usually from different limitations such as insufficient enantioselectivity, low activity, and smallscale synthesis. In addition, some of these chemicals are generally extremely expensive, unstable, and toxic. Asymmetric reduction of the cheap prochiral ketones to the corresponding chiral secondary alcohols with biocatalyst has emerged as the most useful method, and this technique has a high catalytic effect, mild reaction conditions, high selectivity, and being avoid of toxic chemicals. However, enantioselective reduction of aryl heteroaryl ketones in the presence of biocatalyst is still challenging [10, 11]. Diaryl ketones are usually known as “hard-to-reduce” substances for biocatalyst owing to their big steric obstacle and similar of two aromatic groups [12, 13]. Recently, biocatalysts have been shown to be extremely enantioselective in the bioreduction of ketones bearing some large groups [14–16]. Biocatalytic asymmetric reduction occurs as an alternative method for the reduction of aryl heteroaryl ketones, which can be difficult with chemical catalysts [17]. In this way, many studies have been reported, including asymmetric reduction of aromatic ketones and heteroalkyl ketones using biocatalysts [18–22]. However, there is no study involving the asymmetric reduction of phenyl (thiophen-2-yl) methanone 1, which have sulfur atom, using biocatalyst. Sulfur-containing organic compounds play key roles in many biological structures and functions, such as amino acids, and some of the drugs contain sulfur in their structure. Sulfur is a common component of pharmaceuticals, and its selective combination into