The increase in urbanization and industrialization has led to the discharge of wastes in environment causing more pollution. Textile industries are playing a havoc role in water pollution natural aquatic ecosystems. Bioremediation is the one of the cost effective and ecofriendly achievement of biotechnological novelty. Bioremediation is the key to waste management. It can remove textile dyes that are considered to be heterologous biological compounds and are difficult to degrade. In the present bio-remedial method, Klebsiella pneumoniae GM-04 was used promising microbial tool for the decolorization of disperse blue 284 dye. Klebsiella pneumoniae was isolated from industrial wastewater. Under optimum conditions (37 °C and pH 7) Klebsiella pneumoniae led to 95% decoloration of DB-284 (200 ppm) within 24 hrs. The increase in dye concentration and temperature decreased the bacterial growth results in low decoloration of dye. UV–Vis and FTIR analysis suggested that the parent dye degraded into different products. The data was statistically analyzed.