The best microbial species for such an application was selected amongst the isolated
bacterial populations by conducting methylene blue (MB) batch degradation studies with the
bacterial strains using NaCl-yeast as a nutrient medium. The most suitable bacterial species
was Alcaligenes faecalis (A. faecalis) according to 16S rDNA sequencing. Process
parameters were optimized and under the optimum conditions (eg, inoculum size of 3 mL …