The lignocellulosic composition and availability of banana pseudostem is giving the selective choice for the renewable use of it from waste to valued product. The aim of the study was to improve bioethanol production by using separate and cocultures of cellulase enzyme with Saccharomyces cerevisiae. The raw material contains of 42.35% cellulose, 22.63% hemicellulose, 15.36% lignin, 8.79% extractives and 18.70% ash. Hot compressed water (HCW) and dilute H₂SO₄ pretreatment was performed to break down the lignin structure. Cellulase enzyme Aspergillus niger broken the β-glycosidic bonds and delivered the fermentable sugar, which was fermented to ethanol. The fermentation was carried out on the hydrolysate by cultured yeast (S. cerevisiae from grapes). The coculture fermentation in Simultaneous saccharification and fermentation (SSF) was found to be promising with maximum yield of ethanol (8.28 and 5.96 g L^-1 of BP pretreated at 200°C and 0.5% of H₂SO₄, respectively) at 48 h than as compared to the separate hydrolysis and fermentation (SHF). The apparent result of the study observed that highest yield of ethanol was attained in a shorter duration in the coculture system containing cellulase enzyme and S. cerevisiae of grapes.