Formation of biofilm is one of the major protective mechanisms of survival of microbes under
stressed conditions. It is regulated by the quorum sensing (QS) and phenazine (phz) gene.
The phenolic and iron chelators compounds negatively impacts the biofilm formation. They
exert anti-QS activity and disrupt biofilm forming genes. Mutations in oprD gene (an
imipenem resistance factor), Psl, alginate, and Pel genes make bacteria lose their ability to
form biofilms. Similarly, C-di-GMP is the positive regulator of biofilm formation. It plays an …