Heavy metals are the most hazardous pollutants present in industrial and domestic waste water. Microbe related technologies may provide an alternative method of metal removal. The goal of present study was to isolate heavy metal tolerant microorganisms and studied their heavy metal resistance potential. Heavy metal tolerant microorganism was isolated from sewage water collected from different localities (domestic, industrial and agricultural site) of Jabalpur, India. In present study, out of twenty five, only 4 bacterial isolates shows heavy metal resistance potential. These selected isolates were identified as Bacillus carotarum, Bacillus cereus, Bacillus lentus and Bacillus licheniformis according to Bergey’s manual of systemic bacteriology and PIB computer kit by using Bacillus identification matrix. Identity of the isolates was further confirmed at genetic level by DNA homology. Heavy metal tolerance test showed maximum microbial tolerance to Pb and minimum tolerance to Zn and Cr. Our results strongly recommended that the isolated Bacillus sp. has the properties to resist a wide range of heavy metals and antibiotics. These heavy metals may be harmful to human being as well as to the animals. The present investigation has widened the scope for research and development of metal tolerance and antibiotic resistance from bacterial origin. This heavy metal resistant organism could be a potential agent for bioremediation of heavy metals polluted environment.