Synthesis of fungal-derived silver nanoparticles (AgNPs) by endophytic fungi is being of major interest and applied recently in various fields. The current study aims to green synthesis of AgNPs by some endophytic native taxa isolated from six medicinal plants from arid South Sinai and optimizing production conditions combined with photostimulation. Thirteen species out of twenty-two entophytic fungi were screened for production of AgNPs. Reaction conditions such as silver nitrate concentration, pH, temperature and efficiency of photostimulation using monochromatic red polarized light and UV radiations were optimized and assed for high production of AgNPs. High concentrations of AgNPs were produced by Chaetomiumglobosum and Trichodermaviride recovered from Tanacetumsinaicum and Chiliadenusmontanus respectively. Both fungi showed significantly different response to photostimulation by either red polarized or red LED light. T. viride showed a promising results and significant increase in AgNPs production after photostimulation by monochromatic red polarized light. Application of monochromatic red polarized light in the field of bionanotechnology for enhancing green synthesis of AgNPs would be recommended.