The study was carried to find out the distributional pattern of blood groups of a random population samples from the area of Danyore and Gilgit, Pakistan. The blood samples were taken randomly from population. This study is aimed to determine and find significance valve of blood groups with gender and location. The total of 150 limited blood groups were taken and studied, comprising by 56 males population (37.4%) and 94 females population (62.6%). Among 08 blood groups A+ ve has highest count in the total of 150 Population of district Gilgit while Blood Group B-ve and AB-ve has least. The total number of observation for Blood Group A+ ve were 34 (22.7%), among these 8 was males and remaining 26 females. This shows that among A+ ve Blood Group female were dominate as camper to male. The total number of observation for the Blood Group A-ve were 24 (16%), among these 13 were male and 11 were females. The number of observation for the Blood group B+ ve were 28 (18.7%), among these 12 was males and 16 were females. The total number of observation for the Blood Group B-ve were 7 (4.7%), among these 5 were male and only 2 were females whereas the mean number of investigated for the Blood Group AB+ ve were 21 (14%), among these 7 were male and 14 were females. The total numbers of the observation for the Blood Group AB-ve were 8 (5.2%), among these studied individual 3 were male and 5 were females. The total number of observation for the Blood Group O+ ve were 18 (12%) among these 5 were males and remaining 13 were females similarly the mean observation for the Blood Group O-ve were 10 (6.7%) among these 3 were males and 7 were females.