Disclosed are methods of detecting one or more analytes in a sample by:(1) associating the
sample with a surface that includes an analyte binding agent to result in the immobi lization
of the analytes on the surface;(2) contacting the analyte with a composition that includes at
least one phos phor compound with an affinity for the analyte;(3) forma tion of immobilized
analyte binding agent-analyte-phosphor complexes on the surface;(4) separating unbound
phosphor compounds from the immobilized complexes;(5) detecting a presence or absence …