Objective of this study is to compare the inherent characteristics of natural convection flow inside the canister of spent fuel dry storage system with different backfill gases by utilizing computational fluid dynamics (CFD) code. Four working fluids were selected for comparison study. Helium currently used backfill gas for canister, air, nitrogen, and argon are frequently used as coolant in many heat transfer applications. The results indicate that helium has very distinct conductive behavior and show very weak natural convective flow compared to the others. Argon showed the strongest natural convective flow but also the worst coolability. Air and nitrogen showed similar characteristics to each other. However, due to difference in Prandtl number, nitrogen showed more effective natural convective flow. These results suggest that experimental validation for the nitrogen is needed to investigate the potential coolability other than currently commercially used helium.