At present study, carbon nanotube-graphene (CNT-G) supported PtAu, Au and Pt catalysts were prepared by microwave-assisted synthesis method to investigate the direct liquid-fed sodium borohydride/hydrogen peroxide (NaBH₄/H₂O₂) fuel cell performance. Prepared catalysts were characterized by TGA, XRD, TEM, ICP-OES, cyclic voltammetry and rotating disc electrode (RDE) voltammetry. The catalysts were tested in a single NaBH₄/H₂O₂ fuel cell with 25 cm² active area to evaluate fuel cell performance. The effects of temperature and fuel concentration on fuel cell performance were examined to observed best operating conditions. As a result of direct NaBH₄/H₂O₂ fuel cell experiments, maximum power densities of 139 mW/cm², 125 mW/cm² and 113 mW/cm² were obtained for PtAu/CNT-G, Au/CNT-G and Pt/CNT-G catalysts, respectively. PtAu/CNT-G catalyst showed the enhanced NaBH₄/H₂O₂ fuel cell performance, which was higher than the Pt/CNT-G catalyst and Au/CNT-G catalyst at 50 °C. The enhanced NaBH₄/H₂O₂ performance can be attributed to synergistic effects between Pt and Au particles on CNT-G support providing a better catalyst utilization and interaction. These results suggest that the prepared PtAu/CNT-G catalyst is a promising anode catalyst for NaBH₄/H₂O₂ fuel cell application.